Adrenal Neutral Cholesteryl Ester Hydrolase: Identification, Subcellular Distribution, and Sex Differences
نویسندگان
چکیده
منابع مشابه
Adrenal Neutral Cholesteryl Ester Hydrolase: Identification, Subcellular Distribution, and Sex Differences.
Adrenals express a high level of neutral cholesteryl ester hydrolase (CEH) activity, and male rats have greater activity than females; however, the identity of the enzyme(s) responsible for this activity and the basis for the sex differences are unknown. Using mice in which hormone-sensitive lipase (HSL) was inactivated by homologous recombination (HSL -/-), neutral CEH activity was reduced mor...
متن کاملInhibitors of neutral cholesteryl ester hydrolase.
p-Nitrophenyl N-butyl, N-octyl, and N-dodecyl carbamates and a newly synthesized diethyl phosphate compound were studied as potential inhibitors of the cholesteryl ester hydrolases of Fu5AH rat hepatoma cells. Whole homogenates of Fu5AH cells were used as an enzyme source for the assay of cholesteryl ester hydrolase activity. All four compounds led to marked inhibition (70-80%) of neutral chole...
متن کاملTissue and species differences in bile salt-dependent neutral cholesteryl ester hydrolase activity and gene expression.
Enzymatic activity and mRNA abundance for neutral bile salt-dependent cholesteryl ester hydrolase (CEH) were determined in rat and rabbit tissues. In rat liver and intestine, enzyme activity and mRNA levels varied independently. Particularly striking in most tissue samples was the absence of detectable CEH mRNA in the presence of enzymatic activity, suggesting that there was an exogenous source...
متن کاملRegulation of hepatic neutral cholesteryl ester hydrolase by hormones and changes in cholesterol flux.
To understand molecular events in regulation of hepatic neutral cholesteryl ester hydrolase (EC3.1.1.13; CEH), catalytic activity, protein mass, and mRNA levels were measured in rats with various perturbations of hepatic cholesterol metabolism. Cholesterol feeding decreased activity (56 ± 2%), mass (44 ± 2%), and mRNA (14 ± 3%). The cholesterol precursor mevalonate also decreased activity (42 ±...
متن کاملAccelerated Cholesteryl Ester Transfer
To discern the mechanism(s) that underlie abnormal cholesteryl ester transfer (CET) in patients with hypercholesterolemia, we have studied this dysfunctional step in reverse cholesterol transport in 13 subjects with genetically heterogeneous forms of hypercholesterolemia (HC). In all HC patients, the mass of CE transferred in whole plasma from HDL to VLDL and LDL increased rapidly initially and...
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ژورنال
عنوان ژورنال: Endocrinology
سال: 2002
ISSN: 0013-7227
DOI: 10.1210/en.143.3.801